| Product Name | Caspase 8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [8CSP03] to Caspase 8Caspase 8 binds to the death effector domain (DED) of FADD through an analogous DED domain present in tandem in the proform of the Caspase 8 protein. Recruitment of Caspase 8 to the Fas receptor results in oligomerization of the Caspase 8 protein, which in turn drives its autoactivation through "self-cleavage". Activated Caspase 8 then activates other downstream caspases including Caspase 9, thereby, commiting the cell to undergo apoptosis. Reacts with Jurkat cells and Tonsil. |
| Immunogen | Recombinant full length Human Caspase 8 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 8CSP03 |
| Host Species | Mouse |
| Tested Applications | IHC-PIPWB |
| For IP (denatured use protein G): Use at a concentration of 2μg / mg of lysate. For WB: Use at a concentration of 2 - 4 μg / ml for 2hrs at room temperature. For IHC-P: Use at a concentration of 1 - 2 μg / ml for 30 min at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | Q14790 MGC78473 MACH Caspase8 114550 CASP8 MCH5 Caspase 8 841 FLICE ALPS2B CAP4 Casp-8 FLJ17672 Casp 8 |
|---|---|
| Target | Caspase 8 |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | kappa |
| Positive Control | Jurkat Cells, Tonsil |
|---|---|
| Application Notes | For IP (denatured use protein G): Use at a concentration of 2μg / mg of lysate. For WB: Use at a concentration of 2 - 4 μg / ml for 2hrs at room temperature. For IHC-P: Use at a concentration of 1 - 2 μg / ml for 30 min at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH7.4, containing 0.2%BSA and 0.09% sodium azide |
