| Product Name | Caspase 8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [C502S] to Caspase 8Caspase-8, a member of the caspase-family of proteases, plays a key role in mediating Fas (CD95) and TNF induced apoptosis. Caspase-8 is synthesized as inactive pro-enzyme and activation of the enzyme involves proteolytic cleavage that leads to the release of the active p18 and p10 subunits. Activated caspase-8 is able to cleave and activate downstream caspases, such as caspase-3, -6, -7 and a death agonist member of the Bcl-2/Bcl-xL family, Bid, leading to apoptosis. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | C502S |
| Host Species | Mouse |
| Tested Applications | IHC-PIPWB |
| Western blotting (0.5-4 µg/ml) and immunoprecipitation (10 µg/ml). However, the optimal conditions should be determined individually. Detects the 10 kDa small subunit of caspase-8. The antibody does not detect the full-length of caspase-8.: |
|
| Species Reactivity | HumanRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EC 3.4.22.61 MGC78473 MACH procaspase8 CASP-8 procaspase-8 EC3.4.22.61 Caspase8 CASP8 MCH5 ALPS2B FLICE CAP4 Caspase-8 CASP 8 Caspase 8 procaspase 8 Caspase-8 (Active) Active Caspase-8 |
|---|---|
| Target | Caspase 8 |
| Tissue Specificity | This antibody is specific for Caspase-8 (Active). |
| Application Notes | Western blotting (0.5-4 µg/ml) and immunoprecipitation (10 µg/ml). However, the optimal conditions should be determined individually. Detects the 10 kDa small subunit of caspase-8. The antibody does not detect the full-length of caspase-8.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Store at +4C. Product should remain stable for at least 12 months. |
| Storage Buffer | PBS (pH 7.2) containing 50% glycerol, 1% BSA, and 0.02% thimerosal |
