Application
Untreated (–) and treated (+) Jurkat whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 9 (cleaved Asp330) antibody diluted at 1:500.| Product Name | Caspase 9 (cleaved Asp330) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Caspase 9 (cleaved Asp330) (caspase 9, apoptosis-related cysteine peptidase)This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Caspase 9 (cleaved Asp330). The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Caspase 9 |
|---|---|
| Molecular Weight(MW) | 46 kDa (note) (3) |
Application
Untreated (–) and treated (+) Jurkat whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 9 (cleaved Asp330) antibody diluted at 1:500.| Positive Control | Jurkat (10 μM Etoposide treatment for 24 hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
