Application
WB analysis of (A) Jurkat and (B) HeLa lysates using Caspase 9 antibody at a dilution of 1:500.Application
Immunocytochemistry/Immunofluorescence: Caspase 9 Antibody (LAP6 96-2-22) - The Caspase 9 antibody was tested in HeLa cells against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).Application
WB analysis of C6 cell lysate using Caspase 9 antibody [LAP6 96-2-22] at a dilution of 1:1000.Application
IHC-P analysis of human breast carcinoma using Caspase 9 antibody at a dilution of 1:25.Application
Western Blot: Caspase 9 Antibody (LAP6 96-2-22) - The pro and active forms of Caspase 9 detected in active 293 cell lysate (lane 2). Lane 1: inactive 293 cell lysate| Product Name | Caspase 9 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal to human Caspase 9Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase-8 and Caspase-9. Activated initiator caspases then process effector caspases, such as Caspase-3 and Caspase-7, which in turn cause cell collapse. |
| Immunogen | Recombinant human Caspase 9 pro-domain protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | LAP6 96-2-22 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| For WB: Use at a diluton of 1:500 - 1:1,000. Western blot with bands at 46~48 and 35 kDa representing the pro and active forms of Caspase 9, respectively. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 602234 P55211 ICE-LAP6 842 CASPASE9c APAF3 Caspase9 MCH6 CASP9 Caspase 9 CASPASE-9c ICELAP6 APAF-3 APAF 3 ICE LAP6 CASPASE 9c |
|---|---|
| Target | Caspase 9 |
| Tissue Specificity | Specific for Caspase 9. |
| Light Chain Type | kappa |
Application
WB analysis of (A) Jurkat and (B) HeLa lysates using Caspase 9 antibody at a dilution of 1:500.
Application
Immunocytochemistry/Immunofluorescence: Caspase 9 Antibody (LAP6 96-2-22) - The Caspase 9 antibody was tested in HeLa cells against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).
Application
WB analysis of C6 cell lysate using Caspase 9 antibody [LAP6 96-2-22] at a dilution of 1:1000.
Application
IHC-P analysis of human breast carcinoma using Caspase 9 antibody at a dilution of 1:25.
Application
Western Blot: Caspase 9 Antibody (LAP6 96-2-22) - The pro and active forms of Caspase 9 detected in active 293 cell lysate (lane 2). Lane 1: inactive 293 cell lysate| Positive Control | Human kidney 293 cell lysates |
|---|---|
| Application Notes | For WB: Use at a diluton of 1:500 - 1:1,000. Western blot with bands at 46~48 and 35 kDa representing the pro and active forms of Caspase 9, respectively. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Ascitic fluid containing 0.1% sodium azide |
