Application
Sample (30 µg of whole cell lysate) A: MOLT4 5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Caspr2 antibody [N2N3] detects Caspr2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Caspr2 protein stained by Caspr2 antibody [N2N3] diluted at 1:200. Blue: Hoechst 33342 staining.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Caspr2 antibody [N2N3] detects Caspr2n proteins in embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: Caspr2 antibody [N2N3] diluted at 1:250. Blue: DAPI.Application
Caspr2 antibody [N2N3] detects Caspr2 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Caspr2 protein stained by Caspr2 antibody [N2N3] diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .| Product Name | Caspr2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Caspr2 (contactin associated protein-like 2)This gene encodes a member of the neurexin family which functions in the vertebrate nervous system as cell adhesion molecules and receptors. This protein, like other neurexin proteins, contains epidermal growth factor repeats and laminin G domains. In addition, it includes an F5/8 type C domain, discoidin/neuropilin- and fibrinogen-like domains, thrombospondin N-terminal-like domains and a putative PDZ binding site. This protein is localized at the juxtaparanodes of myelinated axons and associated with potassium channels. It may play a role in the local differentiation of the axon into distinct functional subdomains. This gene encompasses almost 1.5% of chromosome 7 and is one of the largest genes in the human genome. It may represent a positional candidate gene for the DFNB13 form of nonsyndromic deafness. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Caspr2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AUTS15 antibody CASPR2 antibody CDFE antibody DKFZp781D1846 antibody NRXN4 antibody PTHSL1 antibody CNTNAP2 antibody contactin-associated protein-like 2 antibody cell recognition molecule Caspr2 antibody contactin-associated protein 2 antibody ho |
|---|---|
| Molecular Weight(MW) | 148 kDa (note) |
| Cellular Localization | Membrane |
Application
Sample (30 µg of whole cell lysate) A: MOLT4 5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Caspr2 antibody [N2N3] detects Caspr2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Caspr2 protein stained by Caspr2 antibody [N2N3] diluted at 1:200. Blue: Hoechst 33342 staining.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Caspr2 antibody [N2N3] detects Caspr2n proteins in embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: Caspr2 antibody [N2N3] diluted at 1:250. Blue: DAPI.
Application
Caspr2 antibody [N2N3] detects Caspr2 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Caspr2 protein stained by Caspr2 antibody [N2N3] diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
