Application
Anti-CaValpha2delta3_(extracellular) - Western blot analysis of rat brain (lanes 1 and 3) and K562 (lanes 2 and 4) lysates: 1, 2. Anti-CaVα2δ3 (extracellular) antibody, (1:200). 3, 4. Anti-CaVα2δ3 (extracellular) antibody preincubated with the control peptide antigen.Application
Anti-CaValpha2delta3_(extracellular) - Expression of CaValpha2delta3 in rat PC12 cells Immunocytochemical staining of intact living PC12 cells. Extracellular staining of cells using Anti-Cavα2δ3 (extracellular) antibody, (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody.Application
Anti-CaValpha2delta3_(extracellular) - Expression of Cavalpha2delta3 in rat hippocampus and cortex Immunohistochemical staining of rat hippocampal CA3 region (A) and rat neocortex (B) using Anti-Cavα2δ3 (extracellular) antibody. In both areas Cavα2δ3 staining (red) appears in pyramidal neurons (arrow). DAPI is used as the counterstain (blue).| Product Name | Cav alpha 2/delta 3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cav alpha 2/delta 3auxillary subinit of the voltage dependent calcium channel; may be involved in calcium current in the heart [RGD, Feb 2006] |
| Immunogen | Peptide CSWWHSDMTAKAQK, corresponding to amino acid residues 942-955 of rat CaVα2δ3 (Accession Q8CFG5). Extracellular. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| ICC/IF:1:200~1:500 IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | calcium channel voltage-dependent alpha2/delta subunit 3 Cacna2d3 |
|---|---|
| Target | Cav alpha 2/delta 3 |
Application
Anti-CaValpha2delta3_(extracellular) - Western blot analysis of rat brain (lanes 1 and 3) and K562 (lanes 2 and 4) lysates: 1, 2. Anti-CaVα2δ3 (extracellular) antibody, (1:200). 3, 4. Anti-CaVα2δ3 (extracellular) antibody preincubated with the control peptide antigen.
Application
Anti-CaValpha2delta3_(extracellular) - Expression of CaValpha2delta3 in rat PC12 cells Immunocytochemical staining of intact living PC12 cells. Extracellular staining of cells using Anti-Cavα2δ3 (extracellular) antibody, (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody.
Application
Anti-CaValpha2delta3_(extracellular) - Expression of Cavalpha2delta3 in rat hippocampus and cortex Immunohistochemical staining of rat hippocampal CA3 region (A) and rat neocortex (B) using Anti-Cavα2δ3 (extracellular) antibody. In both areas Cavα2δ3 staining (red) appears in pyramidal neurons (arrow). DAPI is used as the counterstain (blue).| Application Notes | ICC/IF:1:200~1:500 IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
