Application
Immunohistochemistry was performed on normal biopsies of deparaffinized human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with or without Cav1.1 antibody [1A] overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.| Product Name | Cav1.1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1A] to Cav1.1 |
| Immunogen | Purified rabbit muscle T-tubule dihydropyridine receptor. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1A |
| Host Species | Mouse |
| Tested Applications | BlockingELISAFACSICC/IFIHCIHC-FIHC-PIPWB |
| Recommended Starting Dilutions: IHC-Fr: 1:200. IHC-P: 1:20. FACS: 1ug/test. WB: 1:500. Optimal dilution / concentration should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRatFishGuinea PigRabbit |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | calcium channel voltage-dependent L type alpha 1S subunit CACNA1S |
|---|---|
| Target | Dihydropyridine Receptor alpha-1 |
| Tissue Specificity | This antibody detects Cav1.1 in human, rat, mouse, guinea pig and rabbit skeletal muscle. |
Application
Immunohistochemistry was performed on normal biopsies of deparaffinized human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with or without Cav1.1 antibody [1A] overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.| Application Notes | Recommended Starting Dilutions: IHC-Fr: 1:200. IHC-P: 1:20. FACS: 1ug/test. WB: 1:500. Optimal dilution / concentration should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | PBS contains 0.05 % sodium azide |
