Application
CBP antibody [C3], C-term detects CBP protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. CBP antibody [C3], C-term dilution: 1:750.Application
CBP antibody immunoprecipitates CBP protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of CBP protein by 2.5 μg of CBP antibody 12% SDS-PAGE The immunoprecipitated CBP protein was detected by CBP antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
CBP antibody [C3], C-term detects CBP protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. CBP antibody [C3], C-term dilution: 1:750.Application
CBP antibody [C3], C-term detects CBP protein at nucleus on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. CBP antibody [C3], C-term dilution: 1:750.Application
CBP antibody [C3], C-term detects CBP protein at nucleus on human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. CBP antibody [C3], C-term dilution: 1:750.Application
CBP antibody [C3], C-term detects CBP protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. CBP antibody [C3], C-term dilution: 1:750.Application
Sample (30 µg of whole cell lysate) A: H1299 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
CREBBP antibody immunoprecipitates CREBBP protein-DNA complex in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of CREBBP antibody The precipitated DNA was detected by PCR with primer set targeting to snail promoter.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CBP antibody at 1:200 dilution.| Product Name | CBP antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CBP (CREB binding protein)This gene is ubiquitously expressed and is involved in the transcriptional coactivation of many different transcription factors. First isolated as a nuclear protein that binds to cAMP-response element binding protein (CREB), this gene is now known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. The protein encoded by this gene has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. This protein acetylates both histone and non-histone proteins. This protein shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. Mutations in this gene cause Rubinstein-Taybi syndrome (RTS). Chromosomal translocations involving this gene have been associated with acute myeloid leukemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human CBP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseMonkey |
| Predicted Reactivity | Rat, Rhesus Monkey, BovineRat (95%), Rhesus Monkey (100%), Bovine (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CBP antibody KAT3A antibody RSTS antibody CREBBP antibody CREB-binding protein antibody CREB binding protein antibody |
|---|---|
| Molecular Weight(MW) | 265 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
CBP antibody [C3], C-term detects CBP protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. CBP antibody [C3], C-term dilution: 1:750.
Application
CBP antibody immunoprecipitates CBP protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of CBP protein by 2.5 μg of CBP antibody 12% SDS-PAGE The immunoprecipitated CBP protein was detected by CBP antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
CBP antibody [C3], C-term detects CBP protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. CBP antibody [C3], C-term dilution: 1:750.
Application
CBP antibody [C3], C-term detects CBP protein at nucleus on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. CBP antibody [C3], C-term dilution: 1:750.
Application
CBP antibody [C3], C-term detects CBP protein at nucleus on human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. CBP antibody [C3], C-term dilution: 1:750.
Application
CBP antibody [C3], C-term detects CBP protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. CBP antibody [C3], C-term dilution: 1:750.
Application
Sample (30 µg of whole cell lysate) A: H1299 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
CREBBP antibody immunoprecipitates CREBBP protein-DNA complex in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of CREBBP antibody The precipitated DNA was detected by PCR with primer set targeting to snail promoter.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CBP antibody at 1:200 dilution.| Positive Control | H1299 , *Vero E6 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
