Mouse monoclonal [F4] to CBPProtein Kinase A (PKA) mediated CREB phosphorylation results in the binding of CREB to CBP (CREB-binding protein). Phosphorylation by PKA enables CREB to serve as a scaffold for CBP, which then activates the process responsible for expression of cAMP inducible genes. Sequence analysis of CBP and p300 reveals substantial homology. Reacts with A431 or ML-1 cells and Tonsil.
Immunogen
Purified recombinant CREB-binding protein (CBP)
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
F4
Host Species
Mouse
Tested Applications
ICC/IFIHCIPWB
For IF: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user:
Species Reactivity
HumanMouseRat
Concentration
0.2mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
CBP CREBBP 180849 1387 Q92793 RTS RSTS KAT3A
Target
CBP
Application
Application
Application
Positive Control
A431 or ML-1 Cells, Tonsil
Application Notes
For IF: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.
Storage Buffer
PBS, pH 7.4 containing 0.2% BSA and 0.09% sodium azide