Application
Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE diluted at 1:1000Application
CBX1 / HP1 beta antibody detects CBX1 / HP1 beta protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. CBX1 / HP1 beta antibody diluted at 1:500.Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using CBX1 / HP1 beta antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded 59T xenograft, using CBX1 / HP1 beta antibody at 1:500 dilution.| Product Name | CBX1 / HP1 beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CBX1 / HP1 beta (chromobox homolog 1 (HP1 beta homolog Drosophila ))This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family . The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CBX1 / HP1 beta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Xenopus laevis, Chicken, Chimpanzee, BovineXenopus laevis (80%), Chicken (83%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CBX antibody HP1-BETA antibody HP1Hs-beta antibody HP1Hsbeta antibody M31 antibody MOD1 antibody p25beta antibody CBX1 antibody chromobox homolog 1 (HP1 beta homolog Drosophila ) antibody chromobox protein homolog 1 antibody heterochromatin prote |
|---|---|
| Molecular Weight(MW) | 21 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE diluted at 1:1000
Application
CBX1 / HP1 beta antibody detects CBX1 / HP1 beta protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. CBX1 / HP1 beta antibody diluted at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using CBX1 / HP1 beta antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded 59T xenograft, using CBX1 / HP1 beta antibody at 1:500 dilution.| Positive Control | HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
