Application
CCAR1 antibody detects CCAR1 protein at nucleus on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CCAR1 antibody dilution: 1:500.Application
CCAR1 antibody detects CCAR1 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 5 % SDS-PAGE CCAR1 antibody dilution: 1:1000Application
CCAR1 antibody detects CCAR1 protein at nucleus on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. CCAR1 antibody dilution: 1:500.Application
CCAR1 antibody detects CCAR1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: CCAR1 protein stained by CCAR1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | CCAR1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CCAR1 (cell division cycle and apoptosis regulator 1)Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation (By similarity). May be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CCAR1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Xenopus Tropicalis, Rhesus Monkey, BovineRat (97%), Xenopus laevis (82%), Xenopus Tropicalis (84%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | RP11-437A18.1 cell division cycle and apoptosis regulator 1 |
|---|---|
| Target | CCAR1 |
| Cellular Localization | Cytoplasm , perinuclear region |
Application
CCAR1 antibody detects CCAR1 protein at nucleus on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CCAR1 antibody dilution: 1:500.
Application
CCAR1 antibody detects CCAR1 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 5 % SDS-PAGE CCAR1 antibody dilution: 1:1000
Application
CCAR1 antibody detects CCAR1 protein at nucleus on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. CCAR1 antibody dilution: 1:500.
Application
CCAR1 antibody detects CCAR1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: CCAR1 protein stained by CCAR1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
