Application
ELISA analysis of control antigen and antigen using C-CBL antibody [3B12].Application
WB analysis of human C-CBL (a.a. 684-865) recombinant protein using C-CBL antibody [3B12].Application
WB analysis of (1) RAJI,(2) RAW264.7, (3) K562, (4) SKBR-3, (5) 3T3-L1, (6) THP-1 and (7) PC-12 lysates using C-CBL antibody [3B12].Application
IHC-P analysis of ovarian cancer tissues using C-CBL antibody [3B12].Application
IHC-P analysis of bladder cancer tissues using C-CBL antibody [3B12].Application
ICC/IF analysis of HeLa cells using C-CBL antibody [3B12] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).Application
FACS analysis of MCF-7 cells using C-CBL antibody [3B12] (blue) or a negative control (red).| Product Name | C-CBL antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [3B12] to C-CBLThis gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia. Mutations in this gene are also the cause of Noonan syndrome-like disorder. [provided by RefSeq, Mar 2012] |
| Immunogen | The immunogen corresponding to human C-CBL expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3B12 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | NSLL CBL RNF55 CBL2 FRA11B |
|---|---|
| Target | C-CBL |
| Molecular Weight(MW) | 120 kDa (note) |
Application
ELISA analysis of control antigen and antigen using C-CBL antibody [3B12].
Application
WB analysis of human C-CBL (a.a. 684-865) recombinant protein using C-CBL antibody [3B12].
Application
WB analysis of (1) RAJI,(2) RAW264.7, (3) K562, (4) SKBR-3, (5) 3T3-L1, (6) THP-1 and (7) PC-12 lysates using C-CBL antibody [3B12].
Application
IHC-P analysis of ovarian cancer tissues using C-CBL antibody [3B12].
Application
IHC-P analysis of bladder cancer tissues using C-CBL antibody [3B12].
Application
ICC/IF analysis of HeLa cells using C-CBL antibody [3B12] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).
Application
FACS analysis of MCF-7 cells using C-CBL antibody [3B12] (blue) or a negative control (red).| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
