Application
Flow cytometric analysis of K562 cells using CCT3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of CCT3 Antibody (Center) in MCF7,CEM,K562,HL-60,Hela cell line lysates (35ug/lane).CCT3 (arrow) was detected using the purified Pab.Application
Western blot analysis of CCT3 (arrow) using rabbit polyclonal CCT3 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CCT3 gene (Lane 2) .Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CCT3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | CCT3 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CCT3 (Internal)The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants have been characterized for this gene. In addition, a pseudogene of this gene has been found on chromosome 8. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 305~335 amino acids from the Center region of human CCT3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | TCP1gamma CCT3 CCTgamma Tcomplex protein1 subunit gamma Tcomplex protein 1 subunit gamma hTRiC5 T-complex protein 1 subunit gamma CCTG TCP-1-gamma TRIC5 CCT-gamma CCT gamma TCP 1 gamma |
|---|---|
| Target | CCT3 |
| Molecular Weight(MW) | 60.534 kDa (note) |
Application
Flow cytometric analysis of K562 cells using CCT3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of CCT3 Antibody (Center) in MCF7,CEM,K562,HL-60,Hela cell line lysates (35ug/lane).CCT3 (arrow) was detected using the purified Pab.
Application
Western blot analysis of CCT3 (arrow) using rabbit polyclonal CCT3 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CCT3 gene (Lane 2) .
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CCT3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
