Application
Flow cytometric analysis of K562 cells using CCT3 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of CCT3 Antibody in K562 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CCT3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | CCT3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | T-complex protein 1 subunit gamma, TCP-1-gamma, CCT-gamma, hTRiC5, CCT3, CCTG, TRIC5 |
| Product description | CCT3 is a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin.1) Zebol,J.R., Int. J. Biochem. Cell Biol. 41 (4), 822-827 (2009) |
| Immunogen | This CCT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 509-536 amino acids from the C-terminal region of human CCT3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCT3 |
|---|---|
| Alternative Names | T-complex protein 1 subunit gamma TCP-1-gamma CCT-gamma hTRiC5 CCT3 CCTG TRIC5 |
| Molecular Weight(MW) | 61 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of K562 cells using CCT3 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of CCT3 Antibody in K562 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CCT3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
