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This antibody may be diluted to a titer of 1:20-1:30 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determinedby the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature antigen unmasking is required prior to immunostaining of formalin fixed paraffin embedded tissue sections.:
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