Application
CD10 antibody [N2C1], Internal detects CD10 protein at membrane on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. CD10 antibody [N2C1], Internal dilution: 1:500.Application
CD10 antibody [N2C1], Internal detects MME protein at cytosol and membrane on SkHep1 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SkHep1 xenograft. CD10 antibody [N2C1], Internal dilution: 1:500.Application
CD10 antibody [N2C1], Internal detects MME protein by western blot analysis. A. 50 µg rat kidney lysate/extract 7.5% SDS-PAGE CD10 antibody [N2C1], Internal dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: Jurkat 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
CD10 antibody [N2C1], Internal detects MME protein by western blot analysis. A. 50 µg mouse kidney lysate/extract 7.5% SDS-PAGE CD10 antibody [N2C1], Internal dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | CD10 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CD10 (membrane metallo-endopeptidase)This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CD10. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Rabbit, Rhesus Monkey, BovineDog (89%), Rabbit (88%), Rhesus Monkey (98%), Bovine (86%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CALLA antibody CD10 antibody DKFZp686O16152 antibody MGC126681 antibody MGC126707 antibody NEP antibody SFE antibody MME antibody membrane metallo-endopeptidase variant 2 antibody neprilysin antibody atriopeptidase antibody enkephalinase antibod |
|---|---|
| Molecular Weight(MW) | 86 kDa (note) (4) |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein |
Application
CD10 antibody [N2C1], Internal detects CD10 protein at membrane on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. CD10 antibody [N2C1], Internal dilution: 1:500.
Application
CD10 antibody [N2C1], Internal detects MME protein at cytosol and membrane on SkHep1 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SkHep1 xenograft. CD10 antibody [N2C1], Internal dilution: 1:500.
Application
CD10 antibody [N2C1], Internal detects MME protein by western blot analysis. A. 50 µg rat kidney lysate/extract 7.5% SDS-PAGE CD10 antibody [N2C1], Internal dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: Jurkat 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
CD10 antibody [N2C1], Internal detects MME protein by western blot analysis. A. 50 µg mouse kidney lysate/extract 7.5% SDS-PAGE CD10 antibody [N2C1], Internal dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Jurkat , mouse kidney , rat kidney |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
