Application
Immunofluorescence staining of an infarcted porcine heart with anti-CD105 (MEM-229; green); cell nuclei stained with DAPI (blue).| Product Name | CD105 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Antibody to CD105 (Human) Purified AntibodyEndoglin is a homodimeric transmembrane glycoprotein highly expressed by endothelial cells. It is a component of the transforming growth factor beta receptor complex as it binds TGFB1 and TGFB3 with high affinity.Mutations in the endoglin gene produce hereditary hemorrhagic telangiectasia. |
| Immunogen | recombinant vaccinia virus containing human CD105 (L-isoform) cDNA |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | MEM-229 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| FACS: Recommended dilution: 1:100. Positive control: acute myelogenous leukemia Kg1 cell line.For WB: Suggested starting dilution is 1-2μg/ml under non-reducing conditions.: |
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| Species Reactivity | HumanPig |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | P17813 131195 2022 END ENG ORW CD105 HHT1 ORW1 FLJ41744 |
|---|---|
| Target | CD105 |
| Tissue Specificity | The antibody MEM-229 recognizes human CD105 antigen. |
Application
Immunofluorescence staining of an infarcted porcine heart with anti-CD105 (MEM-229; green); cell nuclei stained with DAPI (blue).| Application Notes | FACS: Recommended dilution: 1:100. Positive control: acute myelogenous leukemia Kg1 cell line.For WB: Suggested starting dilution is 1-2μg/ml under non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
