Application
Sample (30 ug of whole cell lysate) A: THP-1 7.5% SDS PAGE diluted at 1:1000Application
CD11a antibody [N1N3] detects CD11a protein at cell membrane by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD11a protein stained by CD11a antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | CD11a antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CD11a (integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide))ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human CD11a. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | ChimpanzeeChimpanzee (82%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD11A antibody LFA-1 antibody LFA1A antibody ITGAL antibody CD11 antigen-like family member A antibody integrin gene promoter antibody integrin alpha-L antibody leukocyte function-associated molecule 1 alpha chain antibody lymphocyte function-assoc |
|---|---|
| Molecular Weight(MW) | 129 kDa (note) (2) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
Sample (30 ug of whole cell lysate) A: THP-1 7.5% SDS PAGE diluted at 1:1000
Application
CD11a antibody [N1N3] detects CD11a protein at cell membrane by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD11a protein stained by CD11a antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | THP-1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
