Application
Immunoprecipitation of human CD11b/CD18 heterodimer from the lysate of washed PBMC isolated from healthy donor. Lysate was subjected to affinity column chromatography using anti-human CD11b immunosorbent. Eluted immunoprecipitate was resolved on 7.5% SDS-PAGE and stained with Coomasie Blue.| Product Name | CD11b antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM-174] to CD11bCD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. |
| Immunogen | Tissue / cell preparation (Human granulocytes). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | MEM-174 |
| Host Species | Mouse |
| Tested Applications | FACSIPWB |
| Recommended dilutions: FACS: Use at an assay dependant concentration. IP: Immunoprecipitation with the antibody was successfuly performed using immuno-affinity sorbents (minicolumns). The final immunosorbent should contain 1-5 mg of antibody/1 ml of sorbent gel (e.g. agarose beads). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MO1A CD11b 3684 MAC1A ITGAM P11215 MAC1 120980 MAC-1 CR3A MGC117044 MAC 1 |
|---|---|
| Target | CD11b |
Application
Immunoprecipitation of human CD11b/CD18 heterodimer from the lysate of washed PBMC isolated from healthy donor. Lysate was subjected to affinity column chromatography using anti-human CD11b immunosorbent. Eluted immunoprecipitate was resolved on 7.5% SDS-PAGE and stained with Coomasie Blue.| Positive Control | FACS: Peripheral Blood Lymphocytes IP: Granulocytes |
|---|---|
| Application Notes | Recommended dilutions: FACS: Use at an assay dependant concentration. IP: Immunoprecipitation with the antibody was successfuly performed using immuno-affinity sorbents (minicolumns). The final immunosorbent should contain 1-5 mg of antibody/1 ml of sorbent gel (e.g. agarose beads). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
