| Product Name | CD14 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Antibody to CD14 (Human) Purified AntibodyCD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes. |
| Immunogen | Human monocytes |
| Clonality | Monoclonal |
|---|---|
| Clone Number | B-A8 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSIHC-P |
| For ELISA (sandwich): Use at an assay dependent dilution. GTX78343 has been tested as the capture antibody in a sandwich ELISA for analysis of human CD14 in combination GTX78274. For IHC-P: Use at a concentration of 2-10 μg/ml. Positive staining on human PML brain sections was mainly observed on monocytes in the lumenal side of brain blood vessels, and on some perivascular cells adjacent to medium-sized vessels. Heat retrieval of antigen is recommeded. For FACS: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 158120 P08571 929 CD14 |
|---|---|
| Target | CD14 |
| Tissue Specificity | The antibody reacts with CD14, a 53-55 kDa GPI(glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. |
| Positive Control | PML brain sections (IHC-P) |
|---|---|
| Application Notes | For ELISA (sandwich): Use at an assay dependent dilution. GTX78343 has been tested as the capture antibody in a sandwich ELISA for analysis of human CD14 in combination GTX78274. For IHC-P: Use at a concentration of 2-10 μg/ml. Positive staining on human PML brain sections was mainly observed on monocytes in the lumenal side of brain blood vessels, and on some perivascular cells adjacent to medium-sized vessels. Heat retrieval of antigen is recommeded. For FACS: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 15 mM sodium azide |
