Application
Surface staining of human peripheral blood leukocytes using anti-human CD14: FITC .Application
Western Blot analysis (non-reducing conditions) of over-expressed human CD14 using anti-CD14 (MEM-18) . Lane 1: whole cell lysate HEK 293 transfected with empty vector Lane 2: tissue culture supernatant collected after cultivation of HEK 293 transfected with human CD14 cDNA Lane 3: whole cell lysate of HEK 293 transfected with human CD14 cDNA| Product Name | CD14 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Antibody to CD14 (Human) F(ab')2 fragmentThe protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq] |
| Immunogen | A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-18 |
| Host Species | Mouse |
| Tested Applications | FACSWB |
| FACS:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanPrimate |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 158120 P08571 929 CD14 |
|---|---|
| Target | CD14 |
| Tissue Specificity | CD14 antigen (lipopolysaccharide receptor) |
Application
Surface staining of human peripheral blood leukocytes using anti-human CD14: FITC .
Application
Western Blot analysis (non-reducing conditions) of over-expressed human CD14 using anti-CD14 (MEM-18) . Lane 1: whole cell lysate HEK 293 transfected with empty vector Lane 2: tissue culture supernatant collected after cultivation of HEK 293 transfected with human CD14 cDNA Lane 3: whole cell lysate of HEK 293 transfected with human CD14 cDNA| Application Notes | FACS:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Azide free phosphate buffered saline (PBS), preserved by filter sterilization, approx. pH 7.4 |
