Application
Staining of human peripheral blood monocytes with Mouse anti Human CD14:FITCApplication
Western Blot analysis (non-reducing conditions) of over-expressed human CD14 using anti-CD14 (MEM-18) . Lane 1: whole cell lysate HEK 293 transfected with empty vector Lane 2: tissue culture supernatant collected after cultivation of HEK 293 transfected with human CD14 cDNA Lane 3: whole cell lysate of HEK 293 transfected with human CD14 cDNAApplication
Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPEApplication
Surface staining of human peripheral blood leukocytes using anti-human CD14 (clone MEM-18) FITC .| Product Name | CD14 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM - 18] to CD14CD14 is a single-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS)-dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram-negative shock. |
| Immunogen | A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-18 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSIPWBFunctional Assay |
| ELISA: Use at an assay dependant concentration. FACS: Use at an assay dependant concentration. FuncS: Use at an assay dependant dilution. Blocks binding of bacterial LPS to the monocyte surface. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Use under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMonkeyPrimate |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 158120 929 P08571 CD14 |
|---|---|
| Target | CD14 |
| Tissue Specificity | CD14 antigen (human) |
| Cellular Localization | Cytoplasmic. Attached to the membrane by a GPI anchor. |
Application
Staining of human peripheral blood monocytes with Mouse anti Human CD14:FITC
Application
Western Blot analysis (non-reducing conditions) of over-expressed human CD14 using anti-CD14 (MEM-18) . Lane 1: whole cell lysate HEK 293 transfected with empty vector Lane 2: tissue culture supernatant collected after cultivation of HEK 293 transfected with human CD14 cDNA Lane 3: whole cell lysate of HEK 293 transfected with human CD14 cDNA
Application
Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE
Application
Surface staining of human peripheral blood leukocytes using anti-human CD14 (clone MEM-18) FITC .| Application Notes | ELISA: Use at an assay dependant concentration. FACS: Use at an assay dependant concentration. FuncS: Use at an assay dependant dilution. Blocks binding of bacterial LPS to the monocyte surface. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Use under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
