Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using CD14 antibody at 1:500 dilution.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD14 antibody [N2C3] diluted at 1:5000.Application
Sample (30 ug of whole cell lysate) A: K562 10% SDS PAGE diluted at 1:1000| Product Name | CD14 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CD14 (CD14 molecule)CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CD14. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus Monkey, ChimpanzeeRhesus Monkey (97%), Chimpanzee (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD14 antibody myeloid cell-specific leucine-rich glycoprotein antibody monocyte differentiation antigen CD14 antibody CD14 molecule antibody |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) (4) |
| Cellular Localization | Cell membrane , GPI-anchor |
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using CD14 antibody at 1:500 dilution.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD14 antibody [N2C3] diluted at 1:5000.
Application
Sample (30 ug of whole cell lysate) A: K562 10% SDS PAGE diluted at 1:1000| Positive Control | K562 , CD14-transfected 293T cells |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
