Application
Immunofluorescent staining of HT29 cells using anti-BSG mouse monoclonal antibody .Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BSG monoclonal antibody.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BSG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BSG.Application
Anti-BSG mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with BSGApplication
HEK293T cells transfected with either RC219464 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BSG antibody, and then analyzed by flow cytometry.Application
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-BSG mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-BSG mouse monoclonal antibody. ( Dilution 1:50)| Product Name | CD147 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [9H5] to CD147Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. |
| Immunogen | Full length human recombinant protein of human BSG(NP_001719) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 9H5 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| IHC 1:50 : |
|
| Species Reactivity | HumanDogMonkeyRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EMMPRIN OK TCSF BSG 5F7 M6 CD147 |
|---|---|
| Target | BSG |
| Tissue Specificity | is specific for human BSG(NP_001719). |
| Cellular Localization | Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
Application
Immunofluorescent staining of HT29 cells using anti-BSG mouse monoclonal antibody .
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BSG monoclonal antibody.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BSG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BSG.
Application
Anti-BSG mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with BSG
Application
HEK293T cells transfected with either RC219464 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BSG antibody, and then analyzed by flow cytometry.
Application
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-BSG mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-BSG mouse monoclonal antibody. ( Dilution 1:50)| Application Notes | IHC 1:50 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
