| Product Name | CD19 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [BC-3] to CD19CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lamda light chains. It is expressed on approximately 10% of normal human peripheral blood cells and approximately 60% of splenic lymphocytes. It is not expressed on granulocytes, monocytes or T cells as defined by CD3 expression. CD19 defines a pan B antigen which is expressed from the earliest stages of B progenitor development, but is lost on terminal differentiation to plasma cells. It may also be present on some early myeloid progenitors, particularly those of the monoblastic type. The CD19 antigen is expressed on approximately 12% of peripheral blood lymphocytes. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation. It is expressed in all B cell precursor leukemias. Recent cDNA cloning of CD19 has shown significant homology in the cytoplasmic domain of CD19 with the int1 oncogene. |
| Immunogen | Isolated from chronic lymphoid leukemia cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | BC-3 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-F |
| FACS: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. IHC-Fr: 1/5 - 1/20. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | B4 MGC12802 CD19 107265 930 P15391 CVID3 |
|---|---|
| Target | CD19 |
| Cellular Localization | Type I membrane protein. |
| Application Notes | FACS: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. IHC-Fr: 1/5 - 1/20. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide. Constituents: 1% BSA, Ascites, PBS |
