Application
CD19 antibody detects CD19 protein by western blot analysis. A. 30 μg Raji whole cell lysate/extract 7.5 % SDS-PAGE CD19 antibody dilution: 1:1000Application
CD19 antibody [C1C3] detects CD19 protein by flow cytometry analysis. Sample: mouse splenocytes cell fixed in 4% paraformaldehyde at 4ºC for 15 min. Black: Unlabelled sample was used as a control. Red: CD19 antibody [C1C3] dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.Application
CD19 antibody [C1C3] detects CD19 protein at membrane by immunofluorescent analysis. Sample: Raji cells were fixed in ice-cold MeOH for 5 min. Green: CD19 protein stained by CD19 antibody [C1C3] diluted at 1:1000. Blue: Hoechst 33342 staining.Application
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD19 antibody [C1C3] diluted at 1:1000.| Product Name | CD19 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CD19 (CD19 molecule)Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CD19. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Pig, BovinePig (86%), Bovine (82%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | B4 antibody CVID3 antibody MGC12802 antibody CD19 antibody differentiation antigen CD19 antibody B-lymphocyte antigen CD19 antibody B-lymphocyte surface antigen B4 antibody T-cell surface antigen Leu-12 antibody CD19 molecule antibody |
|---|---|
| Molecular Weight(MW) | 61 kDa (note) |
Application
CD19 antibody detects CD19 protein by western blot analysis. A. 30 μg Raji whole cell lysate/extract 7.5 % SDS-PAGE CD19 antibody dilution: 1:1000
Application
CD19 antibody [C1C3] detects CD19 protein by flow cytometry analysis. Sample: mouse splenocytes cell fixed in 4% paraformaldehyde at 4ºC for 15 min. Black: Unlabelled sample was used as a control. Red: CD19 antibody [C1C3] dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
Application
CD19 antibody [C1C3] detects CD19 protein at membrane by immunofluorescent analysis. Sample: Raji cells were fixed in ice-cold MeOH for 5 min. Green: CD19 protein stained by CD19 antibody [C1C3] diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD19 antibody [C1C3] diluted at 1:1000.| Positive Control | Raji , mouse leukocyte |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
