| Product Name | CD2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [B336 ( HuLy - m1 )] to CD2CD2 is a surface antigen of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells (summarized by Sewell et al., 1986 [PubMed 3490670]). It is one of the earliest T-cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it.[supplied by OMIM] |
| Immunogen | Human thymocytes (3 pooled donors). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | B336 (HuLy-m1) |
| Host Species | Mouse |
| Tested Applications | FACSIHC-F |
| For flow cytometry use at 1-5 mg/106 cells. For immunohistochemistry on frozen tissue sections use 10-20 μg/mL. The optimal conditionsshould be determined by the individual lab.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 186990 CD2 914 SRBC T11 P06729 |
|---|---|
| Target | CD2 |
| Tissue Specificity | This antibody reacts with the majority of T-cells in peripheral lymphoid tissue, cortical thymocytes, and most T-cell neoplasms This antibody reacts with the E-rosette receptor on T-cells. This receptor has a molecular weight of approximately 50 kD and is present on the surface of the majority of circulating T-cells. |
| Application Notes | For flow cytometry use at 1-5 mg/106 cells. For immunohistochemistry on frozen tissue sections use 10-20 μg/mL. The optimal conditionsshould be determined by the individual lab.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 1%BSA and 0.09% sodium azide |
