Application
Sample (30 ug of whole cell lysate) A: K562 B: THP-1 12% SDS PAGE diluted at 1:10000Application
CD20 antibody [C2C3], C-term detects CD20 protein at cell membrane by immunofluorescent analysis. Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD20 protein stained by CD20 antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | CD20 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CD20 (membrane-spanning 4-domains, subfamily A, member 1)This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CD20. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Dog, Pig, BovineDog (86%), Pig (86%), Bovine (86%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | B1 antibody Bp35 antibody CD20 antibody CVID5 antibody LEU-16 antibody MGC3969 antibody MS4A2 antibody S7 antibody MS4A1 antibody leukocyte surface antigen Leu-16 antibody B-lymphocyte cell-surface antigen B1 antibody B-lymphocyte antigen CD20 a |
|---|---|
| Molecular Weight(MW) | 33 kDa (note) (2) |
| Cellular Localization | Membrane |
Application
Sample (30 ug of whole cell lysate) A: K562 B: THP-1 12% SDS PAGE diluted at 1:10000
Application
CD20 antibody [C2C3], C-term detects CD20 protein at cell membrane by immunofluorescent analysis. Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD20 protein stained by CD20 antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | K562 , THP-1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
