Application
Western blot analysis of LY75 Antibody (Center) in HL-60 cell line lysates (35ug/lane). LY75 (arrow) was detected using the purified Pab.Application
LY75 Antibody (Center) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human spleen tissue reacted with LY75 Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | CD205 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to LY75 (Internal)Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment (By similarity). Causes reduced proliferation of B-lymphocytes. |
| Immunogen | KLH conjugated synthetic peptide between 653~683 amino acids from the Center region of human LY75. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Lymphocyte antigen75 Ly75 LY75 Lymphocyte antigen 75 Ly75 DEC205 Flags: Precursor CDantigen=CD205 C-type lectin domain family 13 member B Ctype lectin domain family 13 member B gp200MR6 Lymphocyte antigen 75 Ly-75 Ctype lectin domain family13 |
|---|---|
| Target | LY75 |
| Molecular Weight(MW) | 198.297 kDa (note) |
Application
Western blot analysis of LY75 Antibody (Center) in HL-60 cell line lysates (35ug/lane). LY75 (arrow) was detected using the purified Pab.
Application
LY75 Antibody (Center) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human spleen tissue reacted with LY75 Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
