Application
Formalin-fixed and paraffin-embedded human spleen tissue reacted with LY75 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of LY75 Antibody in HL-60 cell line lysates (35ug/lane)| Product Name | LY75 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Lymphocyte antigen 75, Ly-75, C-type lectin domain family 13 member B, DEC-205, gp200-MR6, CD205, LY75, CD205, CLEC13B |
| Product description | LY75 acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment. It causes reduced proliferation of B-lymphocytes.1) Kato,M., et.al., Immunogenetics 47 (6), 442-450 (1998) |
| Immunogen | This LY75 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 646-675 amino acids from the Central region of human LY75. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LY75 |
|---|---|
| Alternative Names | Lymphocyte antigen 75 Ly-75 C-type lectin domain family 13 member B DEC-205 gp200-MR6 CD205 LY75 CD205 CLEC13B |
| Molecular Weight(MW) | 198 kDa |
Application
Formalin-fixed and paraffin-embedded human spleen tissue reacted with LY75 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of LY75 Antibody in HL-60 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
