Application
Sample(30 ug whole cell lysate) A:Raji 7.5% SDS PAGE diluted at 1:5000| Product Name | CD22 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CD22 (CD22 molecule)Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CD22. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ22814 antibody MGC130020 antibody SIGLEC-2 antibody SIGLEC2 antibody CD22 antibody B-lymphocyte cell adhesion molecule antibody T-cell surface antigen Leu-14 antibody sialic acid-binding Ig-like lectin 2 antibody CD22 antigen antibody BL-CAM an |
|---|---|
| Target | CD22 |
| Molecular Weight(MW) | 95 kDa (note) (3) |
| Cellular Localization | Cell membrane |
Application
Sample(30 ug whole cell lysate) A:Raji 7.5% SDS PAGE diluted at 1:5000| Positive Control | Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
