Application
Flow cytometry analysis of CD26 in PC12 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with CD26/DPP4 antibody [236.3] at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated in a proper secondary antibody and re-suspended in PBS for FACS analysis.Application
Immunofluorescent analysis of CD26 (green) in HeLa cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with CD26/DPP4 antibody [236.3] in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a proper secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a magnification of 60x.| Product Name | CD26 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [236.3] to CD26catalyzes the degradation of glucagon-like peptide-1 (Glp-1); involved in proteolysis and peptidolysis [RGD, Feb 2006] |
| Immunogen | Rat 110-120 kD single chain glycoprotein Depeptidyl Peptidase IV (CD26). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 236.3 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIP |
| ICC/IF: 1:20 - 1:200 Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | HumanRat |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | Dpp4 CD26 DPPIV dipeptidylpeptidase 4 |
|---|---|
| Target | CD26/DPP4 |
Application
Flow cytometry analysis of CD26 in PC12 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with CD26/DPP4 antibody [236.3] at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated in a proper secondary antibody and re-suspended in PBS for FACS analysis.
Application
Immunofluorescent analysis of CD26 (green) in HeLa cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with CD26/DPP4 antibody [236.3] in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a proper secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a magnification of 60x.| Application Notes | ICC/IF: 1:20 - 1:200 Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20°C |
| Storage Buffer | PBS |
