Application
Surface staining of human peripheral blood cells with anti-human CD27: PE| Product Name | CD27 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [LT27] to CD27CD27 (50 kDa) is a member of the tumor necrosis factor (TNF) receptor superfamily. CD40 and CD30 are also members of the TNF receptor superfamily. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is a membranebound receptor, but a soluble form of CD27 is also produced. Soluble CD27 is found in body fluids and can be used to monitor local and systemic immune activation. In addition, elevated serum concentrations of soluble CD27 are found in patients with B cell malignancies and soluble CD27 levels strongly correlate with tumor load. |
| Immunogen | Tissue / cell preparation (Human). (Peripheral blood lymphocytes). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | LT27 |
| Host Species | Mouse |
| Tested Applications | FACS |
| FACS: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | TNFRSF7 939 CD27 S152 P26842 MGC20393 186711 Tp55 T14 |
|---|---|
| Target | CD27 |
| Tissue Specificity | CD27 antigen (human). |
| Cellular Localization | Cell Membrane |
Application
Surface staining of human peripheral blood cells with anti-human CD27: PE| Application Notes | FACS: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
