Application
Flow cytometric analysis of PC-3 cells using CD276 mouse mAb ( green) and negative control (purple)Application
Western blot analysis using CD276 mAb against HEK293 (1) and CD276 (AA: 30-130)-hIgGFc transfected HEK293 (2) cell lysateApplication
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues (left) and ovarian cancer tissues (right) using CD276 mouse mAb with DAB staining| Product Name | CD276 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [6A1] to CD276The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| Immunogen | The immunogen corresponding to human CD276 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6A1 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | B7RP-2 4IG-B7-H3 B7-H3 B7H3 |
|---|---|
| Target | CD276 |
| Molecular Weight(MW) | 57 kDa (note) |
Application
Flow cytometric analysis of PC-3 cells using CD276 mouse mAb ( green) and negative control (purple)
Application
Western blot analysis using CD276 mAb against HEK293 (1) and CD276 (AA: 30-130)-hIgGFc transfected HEK293 (2) cell lysate
Application
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues (left) and ovarian cancer tissues (right) using CD276 mouse mAb with DAB staining| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
