Mouse monoclonal antibody [MIH42] to CD276The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
MIH42
Host Species
Mouse
Tested Applications
FACSIHC-F
Flow Cytometry: 1/10 - 1/100:
Species Reactivity
Human
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
B7-H3 CD276 molecule B7H3 4Ig-B7-H3 B7RP-2
Target
CD276
Tissue Specificity
This antibody detects CD276, a member of the B7 family of co-stimulatory molecules also known as B7-H3.
Application
Application
FACS ImageStaining of human peripheral blood monocytes with Mouse anti Human CD276:FITC
Application
FACS ImageStaining of human peripheral blood monocytes with Mouse anti Human CD276:RPE
Application Notes
Flow Cytometry: 1/10 - 1/100:
Additional Information
Form
Liquid
Storage Instructions
Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.