| Product Name | CD28 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [CD28.2] to CD28Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide-MHC complexes, and the second is delivered by accessory or co-stimulating molecules through their counter-receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation. |
| Immunogen | Human CD28 transfected murine T cell hybridoma. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | CD28.2 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-F |
| FACS: Use 5μl for 10^6 cells or 100 μl of whole blood. ICC: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | P10747 186760 Tp44 CD28 MGC138290 940 |
|---|---|
| Target | CD28 |
| Tissue Specificity | recognizes the human CD28 antigen expressed by most T lineage cells. The antibody reacts with human CD28 transfected murine T cell hybridoma, PHA activated blasts and IL 6 dependent plasmacytoma lines. The antibody blocks CD28/B7 (CD80) mediated cell cell adhesion. It binds to the CDR domain in a CDR1 dependent way. may be used for:1. Identification and enumeration of T lymphocytes in peripheral blood.2. T cell isolation by sorting procedures.3. Cell activation studies.4. Cell adhesion studies. |
| Cellular Localization | Type I membrane protein |
| Application Notes | FACS: Use 5μl for 10^6 cells or 100 μl of whole blood. ICC: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 1% BSA, 0.01M PBS. pH 7.4 |
