Application
Formalin fixed paraffin embedded human Hodgkin lymphoma stained with CD30 antibody (cat. # ).| Product Name | CD30 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal to CD30 Ki 1 AntigenThis antibody reacts with a 595 amino acid transmembrane glycoprotein with a molecular weight of 120 kD. Initially designated Ki-1, the CD30 gene was assigned to the short arm of chromosome 1 at position 36.This antibody recognizes Hodgkin's and Reed-Sternberg cells of non-lymphocyte predominant Hodgkin's disease, whereas the tumor cells of lymphocyte predominant Hodgkin's disease show no staining or only weak staining. |
| Immunogen | The Co cell line cells |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | Ber-H2 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-FIHC-P |
| Immunohistochemistry (Paraffin/frozen), Flow Cytometry. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:20 to 1:40 with AutoProbe III (GTX28620) . When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique (see instructions below) is strongly recommended for consistent and reproducible results. The optimal conditions should be determined by the individual laboratory. Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good diluent to use with most antibodies.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P28908 TNFRSF8 943 CD30 KI-1 KI1 D1S166E 153243 KI 1 |
|---|---|
| Target | CD30 |
| Cellular Localization | Cell Membrane |
Application
Formalin fixed paraffin embedded human Hodgkin lymphoma stained with CD30 antibody (cat. # ).| Positive Control | Hodgkins Lymphomas, Large Anaplastic Granulocytes Lymphomas |
|---|---|
| Application Notes | Immunohistochemistry (Paraffin/frozen), Flow Cytometry. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:20 to 1:40 with AutoProbe III (GTX28620) . When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique (see instructions below) is strongly recommended for consistent and reproducible results. The optimal conditions should be determined by the individual laboratory. Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good diluent to use with most antibodies.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, and 0.05% Sodium Azide, pH 7.5. |
