| Product Name | CD30 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [HRS-4] to CD30CD30 is a type I transmembrane glycoprotein of the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkin?s and Reed-Sternberg cells using monoclonal antibody Ki-1. The ligand for CD30 is CD30L (CD153). The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin?s disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin?s and Reed- Sternberg cells, CD30 is also found in some non-Hodgkin?s lymphomas (including Burkitt?s lymphomas), virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that coexpress CD45RO and the IL4 receptor. |
| Immunogen | Hodgkin's derived cell line L540. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | HRS-4 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-PIP |
| Cellular activation: Use at an assay dependent dilution. FACS: Use 2µg for 5X10^5 cells or 100ul of whole blood. IHC-P: 1/50 - 1/100. IP: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | P28908 TNFRSF8 943 CD30 KI-1 KI1 D1S166E 153243 KI 1 |
|---|---|
| Target | CD30 |
| Cellular Localization | Type I membrane protein (long isoform); cytoplasmic (short isoform). |
| Application Notes | Cellular activation: Use at an assay dependent dilution. FACS: Use 2µg for 5X10^5 cells or 100ul of whole blood. IHC-P: 1/50 - 1/100. IP: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: The addition of 0.1% sodium azide is recommended for storage up to one month when stored at 2-8°C.. Constituents: PBS, 1mg/ml BSA |
