Application
IHC-P analysis of human muscle tissue using CD31/PECAM-1 antibody [EPR3094] at a dilution of 1:250.Application
WB analysis of (1) THP-1 and (2) Jurkat lysates (10 μg per lane) using CD31/PECAM-1 antibody [EPR3094] at a dilution of 1:20,000.| Product Name | CD31 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EPR3094] to CD31The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010] |
| Immunogen | Synthetic peptide corresponding to residues in the extracellular domain of human CD31 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EPR3094 |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PIPWB |
| For WB: Use at a dilution of 1:5000 - 1:20000. For IP: Use at a dilution of 1:20. For FACS: Use at a dilution of 1:50. For IHC-P: Use at a dilution of 1:250 - 1:500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. We suggest to program the pressure cooker to run for 30 seconds at 125° C, followed by 10 seconds at 90° C. Then let the slide cool down to room temperature (10 - 20 minutes): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | platelet/endothelial cell adhesion molecule 1 GPIIA' PECAM-1 PECAM1 CD31 endoCAM PECA1 CD31/EndoCAM |
|---|---|
| Target | CD31 |
| Molecular Weight(MW) | 83 kDa (note) |
Application
IHC-P analysis of human muscle tissue using CD31/PECAM-1 antibody [EPR3094] at a dilution of 1:250.
Application
WB analysis of (1) THP-1 and (2) Jurkat lysates (10 μg per lane) using CD31/PECAM-1 antibody [EPR3094] at a dilution of 1:20,000.| Application Notes | For WB: Use at a dilution of 1:5000 - 1:20000. For IP: Use at a dilution of 1:20. For FACS: Use at a dilution of 1:50. For IHC-P: Use at a dilution of 1:250 - 1:500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. We suggest to program the pressure cooker to run for 30 seconds at 125° C, followed by 10 seconds at 90° C. Then let the slide cool down to room temperature (10 - 20 minutes): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% Glycerol, 0.05% BSA and 0.01% Sodium azide. |
