Application
ApplicationApplication
Formalin fixed paraffin embedded human tonsil stained with CD31 antibody (cat. # ).| Product Name | CD31 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [JC / 70A] to CD31CD31 antibodies are a superior marker for angiogenesis, when compared to Factor VIII and CD34 antibodies. CD31 has been used to measure angiogenesis in association with tumor recurrence. CD31 used in a panel with CD34 and Factor VIII has also been used to mark Kaposi's sarcoma and angiosarcomas. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas). |
| Immunogen | Membrane preparation of a spleen from a patient with hairy cell leukaemia. |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | JC/70A |
| Host Species | Mouse |
| Tested Applications | IHCIHC-FIHC-P |
| Immunohistochemistry: use at a dilution of 1 / 10 - 1 / 25 for 30 - 60mins at RT (ABC method). Optimal dilutions / concentrations should be determined by the end user. For formalin fixed paraffin embedded sections, perform enzymatic antigen retrieval using pepsin before commencing with the IHC staining protocol.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | 173445 P16284 5175 PECAM-1 PECAM1 CD31 FLJ34100 FLJ58394 PECAM 1 |
|---|---|
| Target | CD31 |
| Tissue Specificity | This antibody reacts with a 100 kDa glycoprotein expressed by endothelial cells and 130 kDa glycoprotein present in platelets. It stains endothelial cells in normal as well as malignant tissues.all |
| Light Chain Type | kappa |
Application
Application
Application
Formalin fixed paraffin embedded human tonsil stained with CD31 antibody (cat. # ).| Positive Control | Tonsil |
|---|---|
| Application Notes | Immunohistochemistry: use at a dilution of 1 / 10 - 1 / 25 for 30 - 60mins at RT (ABC method). Optimal dilutions / concentrations should be determined by the end user. For formalin fixed paraffin embedded sections, perform enzymatic antigen retrieval using pepsin before commencing with the IHC staining protocol.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | This antibody contains less than 0.1% sodium azide as a preservative. |
