Application
Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with CD31 antibody [N3C2], Internal diluted at 1:10000.Application
CD31 antibody detects CD31 protein at cell membrane of endothelial cells in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. CD31 antibody diluted at 1:250.| Product Name | CD31 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CD31 (platelet/endothelial cell adhesion molecule)Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC). |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CD31. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD31 antibody FLJ34100 antibody FLJ58394 antibody PECAM-1 antibody PECAM1 antibody adhesion molecule antibody CD31 antigen antibody platelet endothelial cell adhesion molecule antibody platelet/endothelial cell adhesion molecule antibody |
|---|---|
| Molecular Weight(MW) | 83 kDa (note) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with CD31 antibody [N3C2], Internal diluted at 1:10000.
Application
CD31 antibody detects CD31 protein at cell membrane of endothelial cells in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. CD31 antibody diluted at 1:250.| Positive Control | HUVEC |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
