Application
Sample (30 µg of whole cell lysate) A: THP-1 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed A431, using CD32B antibody at 1:500 dilution.| Product Name | CD32B antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CD32B (Fc fragment of IgG, low affinity IIb, receptor (CD32))The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CD32B. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500~1:2000 ICC/IF:1:1:00~1:500 |
|
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD32 antibody CD32B antibody FCG2 antibody FCGR2 antibody IGFR2 antibody FCGR2B antibody CDw32 antibody low affinity immunoglobulin gamma Fc region receptor II-b antibody |
|---|---|
| Molecular Weight(MW) | 34 kDa (note) (5) |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
Application
Sample (30 µg of whole cell lysate) A: THP-1 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed A431, using CD32B antibody at 1:500 dilution.| Positive Control | THP-1 |
|---|---|
| Application Notes | WB:1:500~1:2000 ICC/IF:1:1:00~1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
