Application
Surface staining of PHA stimulated human peripheral blood lymphocytes with anti-human CD38: PE .| Product Name | CD38 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [HIT2] to CD38CD38 (T10) is a single chain 46 kDa type II integral glycoprotein with a short N terminal cytoplasmic tail. CD38 is highly expressed on thymocytes. It is also expressed by early cells of B and T lineages, NK cells, plasma cells, monocytes and macrophages and may be detected on cells from multiple myeloma, ALL (B and T) and some AML. In normal lymph nodes and tonsils the antigen is detected mainly on B cells in germinal centers and in plasma cells. The extracellular domain of the molecule shares a high homology sequence with Aplysia ADP ribosyl cyclase. CD38 functions as a multicatalytic ectoenzyme serving as ADP ribosyl cyclase, ADPR hydrolase and possibly NAD+ glycohydrolase, or as a surface receptor. |
| Immunogen | Tissue / cell preparation (Human thymocytes in foetus). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | HIT2 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-FWB |
| FACS: Use at an assay dependant concentration. IHC-Fr: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Use under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 107270 952 T10 P28907 CD38 |
|---|---|
| Target | CD38 |
| Tissue Specificity | CD38 antigen |
| Cellular Localization | Type II membrane protein |
Application
Surface staining of PHA stimulated human peripheral blood lymphocytes with anti-human CD38: PE .| Positive Control | Cell lines derived from B-cell chronic lymphocytic leukemia (B-CLL) |
|---|---|
| Application Notes | FACS: Use at an assay dependant concentration. IHC-Fr: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Use under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
