Application
Sample (30 ug of whole cell lysate) A: K562 10% SDS PAGE diluted at 1:500Application
An ELISA plate is coated with 50 µL of CD4 recombinant protein at concentration ranged from 0.01 µg/mL to 3 µg/mL. The coated protein is detected with CD4 antibody at 0.5 µg/mL.| Product Name | CD4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CD4 (CD4 molecule)This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CD4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus Monkey, ChimpanzeeRhesus Monkey (85%), Chimpanzee (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD4mut antibody CD4 antibody CD4 receptor antibody CD4 antigen (p55) antibody T-cell surface glycoprotein CD4 antibody T-cell surface antigen T4/Leu-3 antibody CD4 molecule antibody |
|---|---|
| Molecular Weight(MW) | 51 kDa (note) |
| Cellular Localization | Cell membrane |
Application
Sample (30 ug of whole cell lysate) A: K562 10% SDS PAGE diluted at 1:500
Application
An ELISA plate is coated with 50 µL of CD4 recombinant protein at concentration ranged from 0.01 µg/mL to 3 µg/mL. The coated protein is detected with CD4 antibody at 0.5 µg/mL.| Positive Control | K562 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
