Application
Sample (30 ug of whole cell lysate) A: THP-1 15% SDS PAGE diluted at 1:2000Application
CD40 antibody detects CD40 protein at cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD40 protein stained by CD40 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using CD40 antibody at 1:250 dilution.| Product Name | CD40 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CD40 (CD40 molecule, TNF receptor superfamily member 5)The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CD40. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Bp50 antibody CDW40 antibody MGC9013 antibody TNFRSF5 antibody p50 antibody CD40 antibody CD40L receptor antibody CD40 antigen (TNF receptor superfamily member 5) antibody B cell-associated molecule antibody CD40 type II isoform antibody B-cell s |
|---|---|
| Molecular Weight(MW) | 31 kDa (note) (4) |
| Cellular Localization | Isoform I: Cell membrane; Single-pass type I membrane protein , Isoform II: Secreted |
Application
Sample (30 ug of whole cell lysate) A: THP-1 15% SDS PAGE diluted at 1:2000
Application
CD40 antibody detects CD40 protein at cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD40 protein stained by CD40 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using CD40 antibody at 1:250 dilution.| Positive Control | K562 , THP-1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
