Mouse monoclonal [156-3C11] to CD44This antibody is specific to a cell surface glycoprotein of 80-95 kD, known as CD44. CD44 is expressed on the surface of lymphocytes, monocytes and granulocytes. This antibody reacts with red cells pretreated with 2-aminoethylisothiouroniumbromide (AET).
Immunogen
Stimulated human leukocytes
Key Feature
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
156-3C11
Host Species
Mouse
Tested Applications
IHCIHC-P
For IHC-P: Use at a dilution of 1:25-1:75 in an ABC method. We suggest an incubation period of 30 minutes at RT. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the researcher.:
Species Reactivity
Human
Concentration
1mg/ml
Purification
Protein A
Target Information
Gene Synonyms
LHR CDW44 CD44 107269 MUTCHI ECMR-III MGC10468 MDU2 ECMRIII Pgp1 IN MUTCH-I MDU3 MIC4 P16070 960 MC56 MUTCH I ECMR III
Target
CD44
Cellular Localization
Cell membrane
Application
Application
IHC image using -Detection of CD44 by IHC in human tonsil
Positive Control
Tonsil
Application Notes
For IHC-P: Use at a dilution of 1:25-1:75 in an ABC method. We suggest an incubation period of 30 minutes at RT. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the researcher.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.