| Product Name | CD44 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [J-173] to CD44CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix. |
| Immunogen | LAZ 221 ALL |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | J-173 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IF |
| FACS: Use 2µg for 5X10^5 cells. IF: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | LHR CDW44 CD44 107269 MUTCHI ECMR-III MGC10468 MDU2 ECMRIII Pgp1 IN MUTCH-I MDU3 MIC4 P16070 960 MC56 CSPG8 HCELL HUTCH-I MUTCH I HUTCH I ECMR III HUTCHI |
|---|---|
| Target | CD44 |
| Tissue Specificity | Leucocytes and erythorocytes. The antibody recognizes a 80-95 kDa transmembrane molecule with extensive glycosylations (O-linked and N-linked). The antibody can immunoprecipitate 3H-Hyaluronate binding activity. This antibody does not inhibit the binding of hyaluronate to the receptor. |
| Cellular Localization | Type I membrane protein |
| Application Notes | FACS: Use 2µg for 5X10^5 cells. IF: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: None. Constituents: PBS, 1mg/ml BSA |
