Application
ApplicationApplication
Immunocytochemistry staining of human peripheral blood mononuclear cell using anti-human CD45 (MEM-28 , green). DNA visualized by DAPI (blue)Application
Surface staining of human peripheral blood cells with anti-human CD45 [MEM-28] (APC).| Product Name | CD45 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM - 28] to CD45CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. |
| Immunogen | Tissue / cell preparation (Human). (Thymocytes and T lymphocytes). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-28 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PIPWB |
| For IHC-P: Use at an assay dependent dilution. No pre-treatment of tissue sections is essential. For WB: Use at a concentration of 2 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | LY5 CD45 T200 5788 PTPRC LCA P08575 B220 126200 GP180 |
|---|---|
| Target | CD45 |
| Tissue Specificity | This product reacts with all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets. |
| Cellular Localization | Type I membrane protein |
Application
Application
Application
Immunocytochemistry staining of human peripheral blood mononuclear cell using anti-human CD45 (MEM-28 , green). DNA visualized by DAPI (blue)
Application
Surface staining of human peripheral blood cells with anti-human CD45 [MEM-28] (APC).| Positive Control | Jurkat human leukemia T-cell lysate and Kg-1a human leukemia T-cell lysate |
|---|---|
| Application Notes | For IHC-P: Use at an assay dependent dilution. No pre-treatment of tissue sections is essential. For WB: Use at a concentration of 2 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline, pH 7.4, containing 15mM sodium azide |
