Mouse monoclonal [4KB5] to CD45RAThe CD45 molecule is comprised of different glycoproteins ranging from 180-240 kD. Expression of CD45 is found on all hemopoietic cells. Detection of the different isoforms can distinguish between different cell forms i.e. naive T cells and memory T cells. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. Their functional activity of each CD45 isotype also varies.
Immunogen
Human hairy cell leukaemia cells.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4KB5
Host Species
Mouse
Tested Applications
IHCIHC-P
For IHC: Use at a dilution of 1:10 - 1:25 (ABC method). We suggest an incubation period of 30 min at RT. Optimal dilutions / concentrations should be determined by the researcher.:
This antibody reacts with most of the B cells in tissue sections and in peripheral blood as well as small population of T cells and monocytes.
Cellular Localization
Cell membrane.
Light Chain Type
kappa
Application
Application
IHC image using - Detection of CD45RA by IHC in human tonsil
Positive Control
Tonsil
Application Notes
For IHC: Use at a dilution of 1:10 - 1:25 (ABC method). We suggest an incubation period of 30 min at RT. Optimal dilutions / concentrations should be determined by the researcher.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.