Application
ApplicationApplication
Western Blot analysis of whole cell lysate of HUT-78 human cutaneous T cell lymphoma cell line. Lane 1: immunostaining with anti-human CD45 control antibody Lane 2: immunostaining with anti-human CD45RA (MEM-56) ; sample of cell lysate prepared under non-reducing conditions Lane 3: immunostaining with anti-human CD45RA (MEM-56) ; sample of cell lysate prepared under reducing conditions| Product Name | CD45RA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM - 56] to CD45RAThe CD45 molecule is comprised of different glycoproteins ranging from 180-240 kD. Expression of CD45 is found on all hemopoietic cells. Detection of the different isoforms can distinguish between different cell forms i.e. naive T cells and memory T cells. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. Their functional activity of each CD45 isotype also varies. |
| Immunogen | Tissue / cell preparation (Human). (Thymocytes and T lymphocytes). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | MEM-56 |
| Host Species | Mouse |
| Tested Applications | FACSIHCIHC-PIPWB |
| For FACS: Use at an assay dependant dilution. For IHC-P: Use at an assay dependant dilution. No pre-treatment of tissue sections is essential. For IP: Use at an assay dependant dilution. For WB: Use at an assay dependant dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | Q14761 CD45 LY5 CD45AP T200 5788 PTPRCAP 601577 PTPRC LCA CD45RA P08575 5790 LPAP B220 126200 GP180 CD45-AP CD45 AP |
|---|---|
| Target | CD45RA |
| Tissue Specificity | CD45RA antigen (human) |
Application
Application
Application
Western Blot analysis of whole cell lysate of HUT-78 human cutaneous T cell lymphoma cell line. Lane 1: immunostaining with anti-human CD45 control antibody Lane 2: immunostaining with anti-human CD45RA (MEM-56) ; sample of cell lysate prepared under non-reducing conditions Lane 3: immunostaining with anti-human CD45RA (MEM-56) ; sample of cell lysate prepared under reducing conditions| Application Notes | For FACS: Use at an assay dependant dilution. For IHC-P: Use at an assay dependant dilution. No pre-treatment of tissue sections is essential. For IP: Use at an assay dependant dilution. For WB: Use at an assay dependant dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline, pH 7.4, containing 15mM sodium azide |
